Supercritical Fluid Chromatographic Separation.

• Yamamoto Shuichi

  Department of Chemical Engineering, Yamaguchi University

Bibliographic Information

Other Title

• 超臨界流体の基礎と応用　超臨界流体によるクロマトグラフィー分離

Abstract

The relationships between the plate height HETP and the linear mobile phase velocity u in supercritical fluid chromatography (SFC) were investigated over a wide range of experimental conditions (pressure p = 8. 5 ∼ 15 MPa, temperature T = 35 ∼ 45°C) with carbon dioxide as the mobile phase. When the HETP - u curves were converted into the dimensionless variables h = HETP/d_p and ν = ud_p/D_m, the curves were similar regardless of the pressure, the temperature and the state of the mobile phase. The distribution coefficient K decreased with increasing T and/or p. The K values were correlated to the mobile phase density. A linear increase of the pressure was applied to elute the sample from the column. The result was simulated by a chromatography model with the isobar chromatography data. These results have shown that the separation behavior of SFC at low sample loadings can be predicted by ordinary liquid chromatography models. Further research should be done for the prediction of the separation behavior at overloaded conditions.

Journal

• The Review of High Pressure Science and Technology

  The Review of High Pressure Science and Technology 5 (2), 78-83, 1996

  The Japan Society of High Pressure Science and Technology

Keywords

• supercritical fluid
• chromatography
• diffusion coefficient
• axial dispersion
• HETP
• distribution coefficient

Details

• CRID: 1390282679357008896
• NII Article ID: 10002848051
• NII Book ID: AN10452913
• DOI: 10.4131/jshpreview.5.78
• ISSN: 13481940
• ISSN: 0917639X
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed