Detailed mapping of the chloroplast genome of barley, Hordeum vulgare L.

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The chloroplast DNA (ctDNA) of barley (<i>Hordeum vulgare L</i>.) was isolated from young green leaves and fragments after partial digestion with <i>Sau</i>3A were cloned into a lambda phage vector. Using an overlapping clone bank of the entire chloroplast genome of barley, we mapped the cleavage sites for the enzymes <i>Xba</i>I, <i>Hin</i>dIII, <i>Pst</i>I, <i>Sal</i>I and <i>Puv</i>II precisely on the circular DNA. The chloroplast genome of barley was found to be 137 kbp long and to contain a pair of inverted repeat regions of 23.7 - 21.1 kbp that separated two single-copy regions of 81.7 - 81.1 kbp and 13.2 - 10.6 kbp, respectively. The restriction map of the barley chloroplast genome showed a high degree of conservation of restriction sites relative to those in the chloroplast genomes of wheat and rye. The genes for 13 transfer RNAs and 33 chloroplast proteins were identified and located on the barley chloroplast genome by hybridization with fragments of rice ctDNA that contained the corresponding genes. The locations of the various genes are very similar to the respective positions on the chloroplast genome of rice and maize.<br>

収録刊行物

  • Genes & genetic systems

    Genes & genetic systems 71(3), 175-180, 1996-06

    The Genetics Society of Japan

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各種コード

  • NII論文ID(NAID)
    10003759864
  • NII書誌ID(NCID)
    AA11077421
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417568
  • NDL 記事登録ID
    4163719
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z53-W539
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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