Genetic analysis of regeneration ability in rice seed-callus

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The inheritance of in vitro shoot regeneration ability in rice seed-callus was studied with the cross combinations between a high and a low regeneration ability cultivar. The mature rice seeds were cultured on 1/2 concentration of MS basal medium containing 3 mg/1 2,4-dichlorophenoxyacetic acid (2,4-D), 5 g/1 yeast extract, 30 g/1 sucrose and ll g/1 agar for callus formation. The formed calli were transferred onto a medium composed of 1/4 concentration of MS plus 1/4 concentration of N<sub>6</sub>, supplemented with 2.5 mg/l 1-naphthaleneacetic acid (NAA), 8 mg/1 kinetin, 30 g/1 sucrose and 3 g/1 gelrite for regeneration. The high regeneration ability cultivars Aikoku and Sen-ichi with regeneration rate of more than 85%, and the low regeneration ability cultivar Moritawase with regeneration rate of no more than 2% were used as cross parents. In F<sub>1</sub> progenies, the regeneration ability appeared similar to their high regeneration ability parents, and there was no difference between reciprocal crosses. F<sub>2</sub> and BC<sub>1</sub> populations showed two distinct patterns of regeneration ability, the high and low regeneration ability plants fitted the 3:1 and 1:1 ratio respectively. The results indicate that the difference of regeneration ability between the high and low parents in this study is controlled by a single dominant gene. The F<sub>2</sub> population of Aikoku × Sen-ichi did not show segregation, suggesting that the dominant gene in these two cutivars is the same allele.<br>

収録刊行物

  • Genes & genetic systems

    Genes & genetic systems 71(5), 313-317, 1996-10

    The Genetics Society of Japan

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各種コード

  • NII論文ID(NAID)
    10003760240
  • NII書誌ID(NCID)
    AA11077421
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417568
  • NDL 記事登録ID
    4163734
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z53-W539
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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