Sequence variation of chloroplast DNA that involves EcoRI and ClaI restriction site polymorphisms in soybean

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Restriction fragment length polymorphisms (RFLPs) of <i>Eco</i>RI- and <i>Cla</i>I-digested chloroplast DNA (cpDNA) within the genus <i>Glycine</i> subgenus <i>Soja</i> were characterized. Two mutations were found to be responsible for the <i>Eco</i>RI and <i>Cla</i>I restriction site polymorphisms, and both were located in a region in which many ribosomal protein genes are clustered. This region is within the large single copy region of cpDNA and is located close to an inverted repeat. The locations of restriction sites of <i>Eco</i>RI and <i>Cla</i>I in the cpDNA region were analyzed by DNA gel-blot analyses and PCR amplification, which were followed by sequencing analyses. The <i>Eco</i>RI site polymorphism was found to have occurred in the intergenic spacer between <i>rps11</i> and <i>rpl36</i>, while the <i>Cla</i>I site polymorphism was located within the 3' part of the coding region of <i>rps3</i>. The mutations that cause <i>Eco</i>RI and <i>Cla</i>I polymorphisms were both found to be single base substitutions. In addition to these polymorphisms, novel sequence variations in soybean cpDNA were detected near the sites of these mutations. Previously, it was shown that cultivated soybeans could be classified into three groups (I, II, and III) based on their cpDNA RFLPs. A comparison of the cpDNA sequences of soybeans in the present study was consistent with the notion that the cpDNA of group II soybeans is an intermediate between the cpDNAs of groups I and III.<br>

収録刊行物

  • Genes & genetic systems

    Genes & genetic systems 73(2), 111-119, 1998-04

    The Genetics Society of Japan

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各種コード

  • NII論文ID(NAID)
    10003762301
  • NII書誌ID(NCID)
    AA11077421
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417568
  • NDL 記事登録ID
    4514156
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z53-W539
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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