Evolutionary relationships among Japanese pond frogs inferred from mitochondrial DNA sequences of cytochrome b and 12S ribosomal RNA genes

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The evolutionary relationships among Japanese pond frogs (<i>Rana nigromaculata</i>, <i>R. porosa porosa</i>, and <i>R. p. brevipoda</i>) were investigated by analyzing nucleotide sequences of mitochondrial cytochrome <i>b</i> (<i>cyt b</i>) and 12S rRNA genes. The nucleotide sequences of 444-bp segment of the <i>cyt b</i> gene and 410-bp segment of 12S rRNA gene were determined by the PCR-direct sequencing method using 18 frogs from 13 populations of Japanese pond frogs, and phylogenetic trees were constructed by the neighbor-joining and maximum likelihood methods using <i>R. catesbeiana</i> as an outgroup. The sequenced 444-bp segment of <i>cyt b</i> gene provided 69 variable sites, and the sequenced 410-bp segment of 12S rRNA gene provided 21 variable sites. The numbers of nucleotide substitutions per site of the <i>cyt b</i> gene within ingroup were 0.0022~0.0205 at the populational level, 0.0368~0.0462 at the racial or subspecific level, and 0.1038~0.1244 at the specific level, whereas those of the 12S rRNA gene were 0~0.0074 at the populational or subspecific level, and 0.0378~0.0456 at the specific level. Most nucleotide substitutions within ingroup occurred at the third codon position of the <i>cyt b</i> gene and were silent mutations. High frequencies of transitions relative to transversions were shown at <i>cyt b</i> and 12S rRNA genes within ingroup. The phylogenetic trees constructed from the nucleotide sequences of the <i>cyt b</i> gene showed that after outgroup <i>R. catesbeiana</i> separated from ingroup frogs, ingroup Japanese pond frogs diverged into <i>R. nigromaculata</i> and <i>R. porosa</i>, then the latter diverged into <i>R. p. porosa</i>, <i>R. p. brevipoda</i> (the typical Okayama race), and the Nagoya race of <i>R. p. porosa</i>. The phylogenetic trees constructed from the nucleotide sequences of the 12S rRNA gene also showed distinct divergence between two species, but not any divergence within species.<br>

収録刊行物

  • Genes & genetic systems

    Genes & genetic systems 73(2), 121-133, 1998-04

    The Genetics Society of Japan

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各種コード

  • NII論文ID(NAID)
    10003762333
  • NII書誌ID(NCID)
    AA11077421
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417568
  • NDL 記事登録ID
    4514157
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z53-W539
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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