Cloning of cDNA for a cell wall-bound acid invertase from tomato (Lycopersicon esculentum) and expression of soluble and cell wall-bound invertases in plants and wounded leaves of L. esculentum and L. peruvianum.
-
- Ohyama Akio
- National Research Institute of Vegetables, Ornamental Plants and Tea (NIVOT)
-
- Nishimura Shigeo
- National Research Institute of Vegetables, Ornamental Plants and Tea (NIVOT)
-
- Hirai Masashi
- National Research Institute of Vegetables, Ornamental Plants and Tea (NIVOT)
Bibliographic Information
- Other Title
-
- Cloning of cDNA for a cell wall-bound a
Search this article
Abstract
A cDNA, Wiv-1, for an isozyme of acid invertase (EC 3.2.1.26) was cloned from wounded leaves of tomato (Lycopersicon esculentum). The encoded protein had a basic isoelectric point and strong similarity to the amino acid sequences of plant cell wall-bound invertases. The conserved sequence WECPD that is found in all plant cell wall-bound invertases was also found in the deduced protein. These results suggested that Wiv-1 encoded a cell wall-bound acid invertase of tomato. Wounding increased the levels of mRNAs for soluble and cell wall-bound invertases and the activities of these invertases in leaves of L. esculentum and of a related species, L. peruvianum. The induction of Aiv-1 mRNA for the soluble enzyme in wounded leaves was not very strong, while that of Wiv-1 mRNA for the wall-bound enzyme was prominent. The level of Aiv-1 mRNA reached a maximum 48 h after wounding while that of Wiv-1 mRNA continued to rise for up to 96 h. These findings suggested that the genes for the two isozymes responded independently to wounding. The levels in various organs of Aiv-1 and Wiv-1 mRNAs were higher in L. esculentum than in L. peruvianum. Possible roles of cell wall-bound acid invertase in wound response and in developing plant are discussed.<br>
Journal
-
- Genes & Genetic Systems
-
Genes & Genetic Systems 73 (3), 149-157, 1998
The Genetics Society of Japan
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390001205444699776
-
- NII Article ID
- 10003762456
-
- NII Book ID
- AA11077421
-
- COI
- 1:CAS:528:DyaK1cXmvF2itbg%3D
-
- ISSN
- 18805779
- 13417568
-
- NDL BIB ID
- 4549975
-
- PubMed
- 9794080
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed