Identification of alien chromosomes in a series of Allium fistulosum-A. cepa monosomic addition lines by means of genomic in situ hybridization

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Genomic in situ hybridization (GISH) was used to identify alien chromosomes in a series of eight different Allium fistulosum - A. cepa monosomic addition lines. Biotin-labeled total genomic DNA from shallot (A. cepa L. Aggregatum group) was used as a probe together with an excess amount of unlabeled blocking DNA from the recipient plant, Japanese bunching onion (A. fistulosum L.). Probe hybridization sites were detected by FITC-conjugated avidin and anti-avidin antibody using an epifluorescence microscope. In each mitotic metaphase cell of all the eight types of monosomic addition lines, the alien chromosomes were successfully discriminated from other 16 A. fistulosum chromosomes. Furthermore, no clear exchanges of chromosome segments between A. cepa and A. fistulosum were observed. This finding indicates that in each addition line an entire (unrecombined) A. cepa chromosome is present in an integral diploid background of A. fistulosum.

Genomic in situ hybridization (GISH) was used to identify alien chromosomes in a series of eight different <i>Allium fistulosum</i> - <i>A. cepa</i> monosomic addition lines. Biotin-labeled total genomic DNA from shallot (<i>A. cepa</i> L. Aggregatum group) was used as a probe together with an excess amount of unlabeled blocking DNA from the recipient plant, Japanese bunching onion (<i>A. fistulosum</i> L.). Probe hybridization sites were detected by FITC-conjugated avidin and anti-avidin antibody using an epifluorescence microscope. In each mitotic metaphase cell of all the eight types of monosomic addition lines, the alien chromosomes were successfully discriminated from other 16 <i>A. fistulosum</i> chromosomes. Furthermore, no clear exchanges of chromosome segments between <i>A. cepa</i> and <i>A. fistulosum</i> were observed. This finding indicates that in each addition line an entire (unrecombined) <i>A. cepa</i> chromosome is present in an integral diploid background of <i>A. fistulosum</i>.<br>

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  • Genes & genetic systems

    Genes & genetic systems 73(5), 311-315, 1998-10

    日本遺伝学会

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各種コード

  • NII論文ID(NAID)
    10003763168
  • NII書誌ID(NCID)
    AA11077421
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417568
  • NDL 記事登録ID
    4663327
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z53-W539
  • データ提供元
    CJP書誌  CJP引用  NDL  IR  J-STAGE 
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