Isolation and characterization of the yeast las21 mutants, which are sensitive to a local anestheticum, tetracaine

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We isolated and characterized yeast mutants whose growth is sensitive to a local anestheticum tetracaine and, at the same time, temperature sensitive. These mutants were collectively called <i>las</i> mutants (<i>l</i>ocal <i>a</i>nestheticum <i>s</i>ensitive). The <i>las21</i> mutants were analyzed in this study. The wild type <i>LAS21</i> gene was cloned by exploiting temperature sensitivity of the <i>las21</i> mutants and we found that <i>LAS21</i> encodes ORF YJL062w which has not been analyzed before. Las21p is putative membrane protein belonging to the major facilitator super family containing plural membrane spanning domains. Complete elimination of the <i>LAS21</i> ORF did not kill the cells but made their growth temperature sensitive. Interestingly, the complete loss of the <i>LAS21</i> gene canceled the sensitivity to tetracaine. The ability of the <i>las21</i> mutants to grow at a higher temperature was recovered in the various media containing an osmotic stabilizer or salts. Furthermore, temperature sensitivity of the <i>las21</i> mutants was partially suppressed by introduction of <i>PKC1</i>, encoding protein kinase C, on a high copy vector. We found some genetic interactions between <i>LAS21</i> and Ras/cAMP cascade genes. These results suggest that <i>LAS21</i> defines unkonwn pathway regulating the stress response of yeast.<br>

収録刊行物

  • Genes & genetic systems

    Genes & genetic systems 73(6), 365-375, 1998-12-01

    The Genetics Society of Japan

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各種コード

  • NII論文ID(NAID)
    10003763398
  • NII書誌ID(NCID)
    AA11077421
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417568
  • NDL 記事登録ID
    4681958
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z53-W539
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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