Temperature - Induced Precipitation of Specific DNA Fragments Using DNA - Poly(N - isopropylacrylamide) Conjugate
Soluble conjugates between double stranded DNA and poly(<I>N</I>-isopropylacrylamide) (polyNIPAAm) was synthesized and applied to the selective separation of DNA fragments: the target fragments were connected to the DNA-polyNIPAAm conjugate with the aid of T4 DNA ligase, followed by temperature-induced precipitation. Target DNA was collected from the resultant precipitate in high purity.
- Chemistry letters
Chemistry letters 1999(5), 381-382, 1999-05-05
The Chemical Society of Japan