Temperature - Induced Precipitation of Specific DNA Fragments Using DNA - Poly(N - isopropylacrylamide) Conjugate

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著者

    • UMENO Daisuke
    • Department of Materials Physics and Chemistry, Graduate School of Engineering, Kyushu University
    • MAEDA Mizuo
    • Department of Materials Physics and Chemistry, Graduate School of Engineering, Kyushu University

抄録

Soluble conjugates between double stranded DNA and poly(<I>N</I>-isopropylacrylamide) (polyNIPAAm) was synthesized and applied to the selective separation of DNA fragments: the target fragments were connected to the DNA-polyNIPAAm conjugate with the aid of T4 DNA ligase, followed by temperature-induced precipitation. Target DNA was collected from the resultant precipitate in high purity.

収録刊行物

  • Chemistry letters

    Chemistry letters 1999(5), 381-382, 1999-05-05

    The Chemical Society of Japan

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各種コード

  • NII論文ID(NAID)
    10004341320
  • NII書誌ID(NCID)
    AA00603318
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    03667022
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
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