Temperature-Induced Precipitation of Specific DNA Fragments Using DNA-Poly(<I>N</I>-isopropylacrylamide) Conjugate

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  • Umeno Daisuke
    Department of Materials Physics and Chemistry, Graduate School of Engineering, Kyushu University
  • Maeda Mizuo
    Department of Materials Physics and Chemistry, Graduate School of Engineering, Kyushu University

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Soluble conjugates between double stranded DNA and poly(N-isopropylacrylamide) (polyNIPAAm) was synthesized and applied to the selective separation of DNA fragments: the target fragments were connected to the DNA-polyNIPAAm conjugate with the aid of T4 DNA ligase, followed by temperature-induced precipitation. Target DNA was collected from the resultant precipitate in high purity.

収録刊行物

  • Chemistry Letters

    Chemistry Letters 28 (5), 381-382, 1999

    公益社団法人 日本化学会

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