Temperature-Induced Precipitation of Specific DNA Fragments Using DNA-Poly(<I>N</I>-isopropylacrylamide) Conjugate
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- Umeno Daisuke
- Department of Materials Physics and Chemistry, Graduate School of Engineering, Kyushu University
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- Maeda Mizuo
- Department of Materials Physics and Chemistry, Graduate School of Engineering, Kyushu University
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Abstract
Soluble conjugates between double stranded DNA and poly(N-isopropylacrylamide) (polyNIPAAm) was synthesized and applied to the selective separation of DNA fragments: the target fragments were connected to the DNA-polyNIPAAm conjugate with the aid of T4 DNA ligase, followed by temperature-induced precipitation. Target DNA was collected from the resultant precipitate in high purity.
Journal
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- Chemistry Letters
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Chemistry Letters 28 (5), 381-382, 1999
The Chemical Society of Japan
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Details 詳細情報について
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- CRID
- 1390282679553339264
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- NII Article ID
- 10004341320
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- NII Book ID
- AA00603318
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- COI
- 1:CAS:528:DyaK1MXjtVGmu7s%3D
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- ISSN
- 13480715
- 03667022
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed