Direct Cell Counting and Observation of Spacial Distribution of Nitrifiers in Aerobic Biofilms by FISH(fluorescent in situ hybridization).

• ARAKI Nobuo

  Department of Civil Engineering, Nagaoka National College of Technology

• OHASHI Akiyoshi

  Department of Environmental System Engineering, Nagaoka University of Technology

• HARADA Hideki

  Department of Environmental System Engineering, Nagaoka University of Technology

• MACHDAR Izarul

  Department of Environmental System Engineering, Nagaoka University of Technology

Bibliographic Information

Other Title

• ＦＩＳＨ法を適用した生物膜内硝化細菌の菌数計速と空間分布の観察
• FISH法を適用した生物膜内硝化細菌の菌数計測と空間分布の観察
• FISHホウ オ テキヨウ シタ セイブツ マク ナイショウカ サイキン ノ キンスウ ケイソク ト クウカン ブンプ ノ カンサツ

Abstract

Abundance and spacial distribution of nitrifying bacteria in aerobic biofilm consortia were investigated by using fluorescent in situ hybridization (FISH) with 16SrRNA-targeted oligonucleotide probes. The biofilms were developed in a novel reactor named downflow hanging sponge-cubes (DHS), which was employed as a cost-effective aerobic post-treatment unit after an UASB anaerobic pre-treatment unit receiving municipal sewage. Microscopic direct counting of nitrifying bacteria detected by the FISH technique was carried out to disintegrated biofilm samples, which were obtained by squeezing the sponge-cubes at different reactor height locations. The cell enumeration suggested that the DHS biomass contained 1.4×10⁹-1.2×10⁸ cells·ml^-1 cube of Nitrosomonas and 1.8×10⁸-0.15×10⁸ cells·ml^-1-cube of Nitrobacter, giving the presence ratio to total cells (detected by DAPI-staining) at 1.4% and 0.18%, respectively. Cell concentrations of both nitrifying bacteria were well correlated with ammonia-oxidizing and nitrite-oxidizing activities evaluated by batch tests. Application of the FISH technique to thinsectioned intact biofilm revealed that both Nitrosomonas and Nitrobacter were rather present in the interior space of sponge-cubes, as attached onto sponge materials. A relatively small amount of Nitrosomonas was observed only until 50μm-depth outer layer of the thick biofilm formed on the surface of sponge-cubes.

Journal

• Journal of Japan Society on Water Environment

  Journal of Japan Society on Water Environment 22 (2), 152-159, 1999

  Japan Society on Water Environment

Keywords

• wastewater treatment
• nitirifying biofilm
• fluorescent <i>in situ</i> hybridization
• <i>Nitrosomonas</i>
• microscopic direct counts

Details

• CRID: 1390282680058947840
• NII Article ID: 10004451761
• NII Book ID: AN10372439
• DOI: 10.2965/jswe.22.152
• COI: 1:CAS:528:DyaK1MXit12mt78%3D
• ISSN: 18813690
• ISSN: 09168958
• ISSN: http://id.crossref.org/issn/09168958
• NDL BIB ID: 4654555
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4654555
• Web Site: https://www.jstage.jst.go.jp/article/jswe/22/2/22_2_152/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed