微小電極およびFISH法を用いた都市下水生物膜内のアンモニア酸化機構の検討 Ammonium Oxidation in Mixed-population Biofilms Determined with the Use of Microelectrodes and in situ Hybridization

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著者

    • 佐藤 久 SATOH Hisashi
    • 北海道大学大学院工学研究科都市環境工学専攻 Depertment of Urban and Environmental Engineering Hokkaido University
    • 岡部 聡 OKABE Satoshi
    • 北海道大学大学院工学研究科都市環境工学専攻 Depertment of Urban and Environmental Engineering Hokkaido University

抄録

Ammonium oxidation in wastewater biofilms grown on rotating biological contactors (RBCs) was investigated by using microelectrodes and fluorescent in situ hybridization (FISH) technique. The density of ammonia-oxidizers which formed dense cell cluster was about 5 times higher in the innermost biofilm than in the middle and surface of the biofilm. Due to the presence of ammonia-oxidizers throughout the biofilm, ammonium oxidation zone was expanded with increasing in oxygen penetration depth, which consequently resulted in an increase of the overall ammonium flux into the biofilm. Specific ammonium oxidation rate was dependent upon the density of ammonia-oxidizers. Although more ammonia-oxidizers were detected in the innermost layer, they might be in the dormant state and not contribute ammonium oxidation during the RBC operation because of substrate transport limitation. Furthermore, the fraction of O2 consumption for nitrification to total O2 consumption was in the range of 35% -53% in a medium with ammonium as the sole electron donor, indicating the biofilm consumed a significant amount of oxygen for other than nitrification. It implied that heterotrophs would grow and outcompete nitrifiers in biofilms even though there was no organic carbon source in the medium. In conclusion, the combination of microelectrode measurements and FISH technique made it possible to relate the spatial organization of ammonia-oxidizers with their in situ ammonium oxidation rates in biofilms, which obviously providing more insight into the nitrification process in wastewater biofilm systems.

収録刊行物

  • 水環境学会誌 = Journal of Japan Society on Water Environment  

    水環境学会誌 = Journal of Japan Society on Water Environment 22(3), 206-214, 1999-03 

    公益社団法人 日本水環境学会

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各種コード

  • NII論文ID(NAID)
    10004451898
  • NII書誌ID(NCID)
    AN10372439
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    09168958
  • NDL 記事登録ID
    4682937
  • NDL 雑誌分類
    ZN5(科学技術--建設工学・建設業--都市工学・衛生工学)
  • NDL 請求記号
    Z16-1045
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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