ミクロシスチンの簡易測定手法に関する研究 Study on Rapid Analysis for Microcystin

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The fast analysis of microcystin in environmental water was examined by using a protein phosphatase 2A inhibition assay. The heat treatment method was developed for rapid extraction of microcystin from Microcystis cells.Quantitative analysis of microcystin by the protein phosphatase 2A inhibition assay required two hours. The assay was accurate and reproducible. The cultured Microcystis suspension was submitted to the protein phosphatase 2A inhibition assay as well as commercial ELISA assay kit with similar results. But sensitivity of this assay was not so good as the ELISA assay kit.The microcystins were extracted from Microcystis cells by heat conditions such as temperature of 50-60 C° and heating time of more than 20 minutes. The heat treatment with α-amylase, cellulase and lysozyme was slightly facilitated to extraction of it.Uses of the protein phosphatase 2A inhibition assay with the heat treatment method facilitate investigation of microcystin in natural water.

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  • 水環境学会誌 = Journal of Japan Society on Water Environment  

    水環境学会誌 = Journal of Japan Society on Water Environment 22(3), 222-227, 1999-03 

    公益社団法人 日本水環境学会

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各種コード

  • NII論文ID(NAID)
    10004451944
  • NII書誌ID(NCID)
    AN10372439
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    09168958
  • NDL 記事登録ID
    4682965
  • NDL 雑誌分類
    ZN5(科学技術--建設工学・建設業--都市工学・衛生工学)
  • NDL 請求記号
    Z16-1045
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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