FISH法を適用した包括固定化担体内でのアンモニア酸化細菌の動態解析 Quantitative Monitoring of Ammonia Oxidizing Bacteria in PVA-immobilized Pellets by Fluorescent in situ Hybridization (FISH)

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著者

    • 荒木 信夫 ARAKI Nobuo
    • 長岡工業高等専門学校環境都市工学科 Department of Civil Engineering, Nagaoka National College of Technology
    • 矢澤 賢一郎 YAZAWA Kenichiro
    • 三機工業(株) 環境システム事業部企画開発部開発課 Sanki Engineering Co., Ltd., Environmental System Administration Division
    • 原田 秀樹 HARADA Hideki
    • 長岡技術科学大学工学部環境システム工学系 Department of Environmental System Engineering, Nagaoka University of Technology

抄録

Dynamic behavior of Nitrosomonas cells immobilized in PVA gel pellets was investigated by fluorescent in situ hybridization (FISH) of oligonucleotide probes. Two laboratory-scale reactors were seeded with PVA-immmobilized Nitrosomonas cells, and then operated for 160 days by feeding with either a synthetic wastewater containing ammonium solely or a synthetic wastewater composed of ammonium and organic source (acetate). The nitrogen loading on the first reactor (organic-free) was varied from 0.2 up to 0.6kgN·m-3·d-1 by stepwise reducing hydraulic retention time, while the second reactor was operated by varying the C/N ratio of the feed from 0 to 0.8.The first reactor (organic-free) achieved 99% of ammonia removal at 300mg-N·l-1·d-1, and ammonia-oxidation activity per unit pellet volume remained unchanged above 4.5 mg-N·ml-1·d-1 throughout 160 days operation. On the other hand, a severe deterioration in ammmonia oxidation was observed in the second reactor (organic-fed) when the feed C/N ratio was increased to 0.8. For the both reactors, the presence ratio of Nitrososnonas cells to DAPI-stained total cells decreased to below 2% within the initial 90 days operation, regardless of the pressence of organic source in the feed. Nitrosomonas cells were present only up to 50μm-depth of the pellets in the form of large cell clusters. The cluster-forming growth of Nitrosomonas tended to break the lattice-structure of PVA gel material, causing the disappearance of these bacteria from the pellets.

収録刊行物

  • 水環境学会誌 = Journal of Japan Society on Water Environment  

    水環境学会誌 = Journal of Japan Society on Water Environment 22(7), 600-607, 1999-07 

    公益社団法人 日本水環境学会

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各種コード

  • NII論文ID(NAID)
    10004452917
  • NII書誌ID(NCID)
    AN10372439
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    09168958
  • NDL 記事登録ID
    4802450
  • NDL 雑誌分類
    ZN5(科学技術--建設工学・建設業--都市工学・衛生工学)
  • NDL 請求記号
    Z16-1045
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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