書誌事項
- タイトル別名
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- Quantitative Monitoring of Ammonia Oxidizing Bacteria in PVA-immobilized Pellets by Fluorescent in situ Hybridization(FISH).
- FISHホウ オ テキヨウ シタ ホウカツ コテイカ タンタイ ナイ デ ノ アンモニア サンカ サイキン ノ ドウタイ カイセキ
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Dynamic behavior of Nitrosomonas cells immobilized in PVA gel pellets was investigated by fluorescent in situ hybridization (FISH) of oligonucleotide probes. Two laboratory-scale reactors were seeded with PVA-immmobilized Nitrosomonas cells, and then operated for 160 days by feeding with either a synthetic wastewater containing ammonium solely or a synthetic wastewater composed of ammonium and organic source (acetate). The nitrogen loading on the first reactor (organic-free) was varied from 0.2 up to 0.6kgN·m-3·d-1 by stepwise reducing hydraulic retention time, while the second reactor was operated by varying the C/N ratio of the feed from 0 to 0.8.<br>The first reactor (organic-free) achieved 99% of ammonia removal at 300mg-N·l-1·d-1, and ammonia-oxidation activity per unit pellet volume remained unchanged above 4.5 mg-N·ml-1·d-1 throughout 160 days operation. On the other hand, a severe deterioration in ammmonia oxidation was observed in the second reactor (organic-fed) when the feed C/N ratio was increased to 0.8. For the both reactors, the presence ratio of Nitrososnonas cells to DAPI-stained total cells decreased to below 2% within the initial 90 days operation, regardless of the pressence of organic source in the feed. <br>Nitrosomonas cells were present only up to 50μm-depth of the pellets in the form of large cell clusters. The cluster-forming growth of Nitrosomonas tended to break the lattice-structure of PVA gel material, causing the disappearance of these bacteria from the pellets.
収録刊行物
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- 水環境学会誌
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水環境学会誌 22 (7), 600-607, 1999
公益社団法人 日本水環境学会
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詳細情報 詳細情報について
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- CRID
- 1390282680056832640
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- NII論文ID
- 10004452917
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- NII書誌ID
- AN10372439
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- COI
- 1:CAS:528:DyaK1MXlslGksbY%3D
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- ISSN
- 18813690
- 09168958
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- NDL書誌ID
- 4802450
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
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- 使用不可