FISH法を用いた都市下水生物膜内における硝化細菌の動態解析 Population Dynamics of Nitrifying Bacteria in Wastewater Biofilms Analyzed by Fluorescent in situ Hybridization

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著者

    • 岡部 聡 OKABE Satoshi
    • 北海道大学大学院工学研究科都市環境工学専攻 Department of Urban and Environmental Engineering, Graduate School of Engineering, Hokkaido University
    • 内藤 初夏 [他] NAITOH Hatsuka
    • 北海道大学大学院工学研究科都市環境工学専攻 Department of Urban and Environmental Engineering, Graduate School of Engineering, Hokkaido University
    • 渡辺 義公 WATANABE Yoshimasa
    • 北海道大学大学院工学研究科都市環境工学専攻 Department of Urban and Environmental Engineering, Graduate School of Engineering, Hokkaido University

抄録

Time dependent developments of in situ spatial distributions of NH4+- and NO2-- oxidizing bacterial populations in domestic wastewater biofilms and in synthetic nitrifying biofilms were investigated by fluorescent in situ hybridization (FISH) with a set of 16S rRNA-targeted oligonucleotide probes. NH4+-oxidizing bacterial populations were classified into three groups according to the probe specificity, and population dynamics of these groups was correlated with the biofilm performance. In situ hybridization indicated that Nitrosomonas spp. (excluding probe NEU stained NH4+-oxidizing bacteria : i.e., N. marina-lineage, N. europaea-lineage, N. eutropha, and N. halophila) and Nitrospira-like bacteria were the numerically dominant species in the domestic wastewater biofilms. However, probe NEU stained NH4+-oxidizing bacteria became dominant populations in the synthetic nitrifying biofilms (which were initially cultured with the primary settling tank effluent) after switching to the synthetic media. This population shift might be attributed to the accumulation of NO2--N and relatively higher loads of NH4+-N which accelerate the growth of N. europaea-lineage and N. eutropha. This evidence indirectly supported that N. europaea has been most commonly isolated and studied in most of the previous researches. These observations have considerable significance to our understanding of microbial nitrification process in wastewater treatment processes and in natural environment.

収録刊行物

  • 水環境学会誌 = Journal of Japan Society on Water Environment  

    水環境学会誌 = Journal of Japan Society on Water Environment 22(8), 683-691, 1999-08 

    公益社団法人 日本水環境学会

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各種コード

  • NII論文ID(NAID)
    10004453102
  • NII書誌ID(NCID)
    AN10372439
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    09168958
  • NDL 記事登録ID
    4834518
  • NDL 雑誌分類
    ZN5(科学技術--建設工学・建設業--都市工学・衛生工学)
  • NDL 請求記号
    Z16-1045
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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