微小電極およびFISH法を用いた生物膜内アンモニア酸化細菌菌体密度分布および活性分布に及ぼす流入基質C/N比の影響評価 Significance of Substrate C/N Ratio on Structure and Activity of Nitrifying Biofilms Determined by In Situ Hybridization and the Use of Microelectrodes

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著者

    • 佐藤 久 SATOH Hisashi
    • 北海道大学大学院工学研究科都市環境工学専攻 Department of Urban and Environmental Engineering, Hokkaido University
    • 岡部 聡 OKABE Satoshi
    • 北海道大学大学院工学研究科都市環境工学専攻 Department of Urban and Environmental Engineering, Hokkaido University

抄録

Effects of substrate C/N ratio on the spatial distributions of ammonia-oxidizing bacterial population and their activities in biofilms were investigated by fluorescent in situ hybridization (FISH) with 16S rRNA-targeted oligonucleotide probes and the use of microelectrodes. The increase in substrate C/N ratio (i.e., addition of acetate) immediately intensified the interspecies competition for oxygen between ammonia-oxidizing bacteria and heterotrophic bacteria, and consequently resulted in deterioration of ammonium oxidation rate especially in the surface of the biofilm. This decrease in the ammonium oxidation rate in the surface could explain a steep spatial gradient of ammonia-oxidizing bacterial population within the biofilms cultured at high substrate C/N ratios.Furthermore, development of the spatial distributions of ammonia-oxidizing bacteria and their activities within the biofilm was followed during biofilm development. The active ammonium oxidation zone gradually shifted from the upper layer to the inner layer of the biofilm, which corresponded with the development of the spatial distributions of ammonia-oxidizing bacteria within the biofilm. Based on in situ analyses of microbial structure and activity, we discussed on mechanisms of population dynamics of ammonia-oxidizing bacteria in biofilms.

収録刊行物

  • 水環境学会誌 = Journal of Japan Society on Water Environment  

    水環境学会誌 = Journal of Japan Society on Water Environment 22(9), 763-769, 1999-09 

    公益社団法人 日本水環境学会

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各種コード

  • NII論文ID(NAID)
    10004453210
  • NII書誌ID(NCID)
    AN10372439
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    09168958
  • NDL 記事登録ID
    4854543
  • NDL 雑誌分類
    ZN5(科学技術--建設工学・建設業--都市工学・衛生工学)
  • NDL 請求記号
    Z16-1045
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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