コッメトアッセイを用いた廃棄物埋立処分場浸出水に含まれるDNA損傷性の検出 Detection of DNA Damaging Ptential in the Leachate from Waste Landfill with Comet Assay

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著者

    • 島崎 大 SHIMAZAKI Dai
    • 東京大学大学院工学系研究科都市工学専攻 Department of Urban Engineering, Division of Engineering, Graduate School of The University og Tokyo

抄録

Application of comet assay (single cell gel electrophoresis assay) to detect DNA damaging potential in the environmental water was examined in this study. Cultured human lymphocyte cell line was served as target organism and contacted in vitro with concentrated leachate samples from a waste landfill. Dose-response relationship was confirmed with positive DNA damaging reagents (B(a)P and 4NQO). Comet assay could detect statistically positive DNA damage in 3-folds concentrated leachate with S9mix metabolic activation. DNA damaging potential in the samples seemed to be caused by ash, which consists most of disposed wastes on the landfill. Differences of detection sensitivity and characteristic between comet assay and conventional microbiological mutagenic tests were also discussed.

収録刊行物

  • 水環境学会誌 = Journal of Japan Society on Water Environment  

    水環境学会誌 = Journal of Japan Society on Water Environment 22(9), 783-789, 1999-09 

    公益社団法人 日本水環境学会

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各種コード

  • NII論文ID(NAID)
    10004453271
  • NII書誌ID(NCID)
    AN10372439
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    09168958
  • NDL 記事登録ID
    4854569
  • NDL 雑誌分類
    ZN5(科学技術--建設工学・建設業--都市工学・衛生工学)
  • NDL 請求記号
    Z16-1045
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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