Tissue Based Enzymatic Determination of ATP in Human Erythrocyte Hemolysate Combined with Spectrophotometric Method

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著者

    • KATAOKA Makoto
    • Laboratory of Analytical Chemistry, Department of Chemistry, University of Tsukuba
    • TESHIMA Norio
    • Laboratory of Analytical Chemistry, Department of Chemistry, University of Tsukuba
    • KURIHARA Makoto
    • Laboratory of Analytical Chemistry, Department of Chemistry, University of Tsukuba
    • KUWA Katsuhiko
    • College of Medical Technology and Nursing, University of Tsukuba
    • KAWASHIMA Takuji
    • Laboratory of Analytical Chemistry, Department of Chemistry, University of Tsukuba

抄録

A potato tissue based enzymatic determination of ATP combined with spectrophotometric detection is proposed. ATP can be hydrolyzed to give AMP via ADP and two moles of orthophosphate by apyrase (EC 3.6.1.5) contained in the potato tissue. The phosphate released is determined spectrophotometrically based on molybdenum blue formation (λ<SUB>max</SUB> = 712 nm). A linear calibration graph is obtained over the range 1.3 × 10<SUP>−6</SUP>–1.3 × 10<SUP>−5</SUP> mol dm<SUP>−3</SUP> ATP with a detection limit of 1.5 × 10<SUP>−7</SUP> mol dm<SUP>−3</SUP> (S/N = 3). The proposed method was successfully applied to the determination of ATP in human erythrocyte hemolysate.

収録刊行物

  • Chemistry letters  

    Chemistry letters 1999(6), 491-492, 1999-06-05 

    The Chemical Society of Japan

参考文献:  13件

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各種コード

  • NII論文ID(NAID)
    10004486167
  • NII書誌ID(NCID)
    AA00603318
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    03667022
  • データ提供元
    CJP書誌  J-STAGE 
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