In vivo Simultaneous Monitoring by Pt - disk Microelectrodes of Intracerebral Hydrogen Peroxide and Dopamine in Rats
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A technique was developed for simultaneous monitoring of temporal changes in hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>) and dopamine (DA) levels by using Pt-disk microelectrodes (φ 30 μm). H<SUB>2</SUB>O<SUB>2</SUB> and DA were determined by differential double-pulse amperometry (for H<SUB>2</SUB>O<SUB>2</SUB>; 1st step, 750 mV, 1000 ms; 2nd step, 1100 mV, 1000 ms: for DA; 1st step, 150 mV, 600 ms; 2nd step, 250 mV, 50 ms). The electrode for H<SUB>2</SUB>O<SUB>2</SUB> or DA is capable of determining each target substance and these substances do not interfere with each other. For an <I>in vivo</I> application, the electrodes were implanted into the striatum of rats and used successfully to monitor intrastriatal changes in H<SUB>2</SUB>O<SUB>2</SUB> and DA simultaneously after intraperitoneal injection of methamphetamine while the animals were moving freely.
- Chemistry Letters
Chemistry Letters 1999(6), 497-498, 1999-06-05
The Chemical Society of Japan