Large Flexibility of Dihydrofolate Reductase as Revealed by Temperature Effects on the Volume and Compressibility

この論文にアクセスする

この論文をさがす

著者

    • OHMAE Eiji
    • Department of Chemistry, Faculty of Science, Hiroshima University
    • GEKKO Kunihiko
    • Department of Chemistry, Faculty of Science, Hiroshima University

抄録

The partial specific volume and adiabatic compressibility of dihydrofolate reductase (DHFR) from <I>Escherichia coli</I> remarkably increased as temperature was higher, corresponding to the conformational changes as revealed by spectroscopic measurements. These results demonstrate that this protein has a highly flexible structure at the native state whose tertiary structure or hydrophobic core is easily expanded with temperature to produce the internal cavities.

収録刊行物

  • Chemistry letters

    Chemistry letters 1999(6), 507-508, 1999-06-05

    The Chemical Society of Japan

参考文献:  11件中 1-11件 を表示

  • <no title>

    KARPLUS M.

    CRC Crit. Rev. Biochem. 9, 293, 1981

    被引用文献2件

  • <no title>

    COOPER A.

    Proc. Natl. Acad. Sci. U.S.A. 73, 2740, 1976

    被引用文献6件

  • <no title>

    GEKKO K.

    J. Phys. Chem. 83, 2706, 1979

    被引用文献7件

  • <no title>

    GEKKO K.

    Biochemistry 25, 6563, 1986

    被引用文献7件

  • <no title>

    OHMAE E.

    J. Biochem. 120, 946, 1996

    被引用文献1件

  • <no title>

    GEKKO K.

    J. Biochem. 116, 34, 1994

    被引用文献1件

  • <no title>

    GEKKO K.

    Protein Sci. 5, 542, 1996

    被引用文献2件

  • <no title>

    OHMAE E.

    J. Biochem. 123, 33, 1998

    被引用文献1件

  • <no title>

    SAWAYA M. R.

    Biochemistry 36, 586, 1997

    被引用文献2件

  • <no title>

    CHALIKIAN T. V.

    J. Mol. Biol. 260, 588-603, 1996

    DOI 被引用文献7件

  • <no title>

    KUWAJIMA K.

    Biochemistry 30, 7693-7703, 1991

    被引用文献13件

被引用文献:  1件中 1-1件 を表示

各種コード

  • NII論文ID(NAID)
    10004486273
  • NII書誌ID(NCID)
    AA00603318
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    03667022
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
ページトップへ