Suitable Experimental Design for Determination of Auxin Polar Transport in Space Using a Spaceecraft

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It is necessary to establish a suitable experimental design for the determination of auxin (indole-3-acetic acid: IAA) polar transport in space using a spacecraft in concerning with the role of gravity. Problems in space experiments are as follows: I) Selection of suitable plant species; II) Preservation of integrity of plant segments for activities of auxin polar transport; III) Stop of auxin polar transport of the segments after the transport experiment in space. Segments of etiolated pea epicotyls and etiolated maize coleoptiles showed relatively high activities of auxin polar transport among dicotyledonous and monocotyledonous plants tested, respectively. The activities decreased dramatically when the segments were pre-stored at 25 °C only for 1 day. On the other hand, the storage at low temperature (5 °C) in the presence of antioxidants or chelating agents, especially EGTA, maintained relatively high activities of auxin polar transport in pea epicotyl segments. Low temperature (5 °C) substantially inhibited the activity of auxin polar transport. Based on the results in this study, a suitable experimental design for the space experiment of auxin polar transport using a spacecraft is also proposed.

収録刊行物

  • 宇宙生物科学 = Biological sciences in space  

    宇宙生物科学 = Biological sciences in space 14(1), 9-13, 2000-03 

    日本宇宙生物科学会

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各種コード

  • NII論文ID(NAID)
    10004491395
  • NII書誌ID(NCID)
    AN10164806
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09149201
  • NDL 記事登録ID
    5361813
  • NDL 雑誌分類
    ZR1(科学技術--生物学) // ZM33(科学技術--宇宙科学)
  • NDL 請求記号
    Z18-2504
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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