液‐液界面における接着性細胞の培養

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  • Cultivation of Adherent Cells on Liquid-Liquid Interface.
  • エキ エキ カイメン ニ オケル セッチャクセイ サイボウ ノ バイヨウ

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The authors proposed a valuable method for the cultivation of adherent cells on an oil-water interface. It was found experimentally that A-375 cell line adhered to a fluorocarbon-medium interface and spread all over the plane interface where there were good or had combinations of oil and water for cell growth. Cultivation on oil droplet surfaces was also studied with a small amount of surface-active agent added, where the percentage of droplets with/without cell adhesion varied with oil droplet diameter and amount of surface-active agent. By the present method, about 90% of cultivated cells was obtained without the usual practice of using trypsin solution to remove cells from the wall surface.

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