高圧力下で誘発される細胞膜の貫通孔 High Pressure-Induced Membrane Tunnel

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著者

    • 加藤 倫子 KATO Michiko
    • 京都大学大学院農学研究科応用生命科学専攻 Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
    • 林 力丸 HAYASHI Rikimaru
    • 京都大学大学院農学研究科応用生命科学専攻 Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

抄録

High pressure treatment of microbial cells and plant and animal tissues at 100-400 MPa solubilizes cellular compounds such as metals, amino acids, and proteins, permeates extra-cellular compounds such as salts into cells and tissues, and causes hemolysis. After the pressure treatment, electron microscopic observation of yeast cells and biochemical analysis of animal tissues reveal a significant membrane breakage of nuclei, lysosomes, and vacuoles in addition to light cell membrane and cell wall damage. To understand these observed phenomena at the molecular level, we studied functional and structural changes of the membrane-bound Na<SUP>+</SUP>, K<SUP>+</SUP>-ATPase under increasing high pressure (in situ observation), and reached an interesting conclusion that contact faces of lipid and membrane-penetrating protein surfaces are reversibly separated to produce tunnels or holes at 100-250 MPa, followed by disordered breakdown of the membrane system including protein denaturation at 300 MPa or higher. This conclusion is well supported by the phase transition of the lipid bilayer membrane.

収録刊行物

  • 高圧力の科学と技術 = The Review of high pressure science and technology  

    高圧力の科学と技術 = The Review of high pressure science and technology 9(3), 183-190, 1999-08-20 

    The Japan Society of High Pressure Science and Technology

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各種コード

  • NII論文ID(NAID)
    10004663880
  • NII書誌ID(NCID)
    AN10452913
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    0917639X
  • NDL 記事登録ID
    4817259
  • NDL 雑誌分類
    ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z17-1589
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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