高圧力バイオサイエンス　　高圧力下で誘発される細胞膜の貫通孔

• 加藤 倫子

  京都大学大学院農学研究科

• 林 力丸

  京都大学大学院農学研究科

書誌事項

タイトル別名

• High Pressure Bioscience. High Pressure-Induced Membrane Tuunel.
• コウアツリョク カ デ ユウハツ サレル サイボウマク ノ カンツウコウ

抄録

High pressure treatment of microbial cells and plant and animal tissues at 100-400 MPa solubilizes cellular compounds such as metals, amino acids, and proteins, permeates extra-cellular compounds such as salts into cells and tissues, and causes hemolysis. After the pressure treatment, electron microscopic observation of yeast cells and biochemical analysis of animal tissues reveal a significant membrane breakage of nuclei, lysosomes, and vacuoles in addition to light cell membrane and cell wall damage. To understand these observed phenomena at the molecular level, we studied functional and structural changes of the membrane-bound Na⁺, K⁺-ATPase under increasing high pressure (in situ observation), and reached an interesting conclusion that contact faces of lipid and membrane-penetrating protein surfaces are reversibly separated to produce tunnels or holes at 100-250 MPa, followed by disordered breakdown of the membrane system including protein denaturation at 300 MPa or higher. This conclusion is well supported by the phase transition of the lipid bilayer membrane.

収録刊行物

• 高圧力の科学と技術

  高圧力の科学と技術 9 (3), 183-190, 1999

  日本高圧力学会

キーワード

• high pressure-treatment
• membrane breakdown
• membrane-bound Na<SUP>+</SUP>
• K<SUP>+</SUP>-ATPase
• high pressure-induced membrane hole

詳細情報

• CRID: 1390282679359006208
• NII論文ID: 10004663880
• NII書誌ID: AN10452913
• DOI: 10.4131/jshpreview.9.183
• ISSN: 13481940
• ISSN: 0917639X
• NDL書誌ID: 4817259
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4817259
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可