長期吸入実験後の肺内に沈着したディーゼル粒子の定量 Quantification of Diesel Exhaust Particles Deposited in the Lungs after Long Term Inhalation
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A method for measuring deposited particles in the lung was investigated, and particle masses deposited in the lungs of rats and guinea pigs from inhaled diesel exhaust were determined. The lung tissues were dissolved with ethanol solution of potassium hydroxide, and the absorbance of solution was measured to determine the deposited particle mass. Determination of elemental carbon required the removal of organic matters from the filtrate of completely digested lung-tissue solution, followed by heat treatment in an inert gas. A high pressure liquid chromatography was applied for quantifying polynuclear aromatic hydrocarbons. The detection limit of particle concentration in the solution was 25 μg/ml. The mass of particles and elemental carbon deposited in the lungs increased with the particle concentration and the inhalation period. Approximately 28 mg and 16 mg of particles were deposited in the lungs of rats at the diesel exhaust particle concentrations of 3.7 mg/m<SUP>3</SUP> and 2.3 mg/m<SUP>3</SUP> for 30 months, and about 64 mg of particles was deposited in the lungs of guinea pigs exposed to the concentration of 2.9 mg/m<SUP>3</SUP> for 24 months. The apparent deposition fraction of diesel particles for each experimental group was in the range between 8 % to 20 % for rats and 10 % to 45 % for guinea pigs. The concentrations of PAHs in the lungs were found to be very low compared to the estimated mass of inhaled PAHs.
- Earozoru Kenkyu
Earozoru Kenkyu 14(4), 329-337, 1999-12-20
Japan Association of Aerosol Science and Technology