生体高分子　　高圧ＮＭＲによる蛋白質研究の新しい展開　　広い構造アンサンブルの探索

• 赤坂 一之

  神戸大学大学院自然科学研究科

書誌事項

タイトル別名

• Biopolymer. High Pressure NMR Enabling a Wide Conformational Search of Proteins.
• コウアツ NMR ニ ヨル タンパクシツ ケンキュウ ノ アタラシイ テンカイ ヒロイ コウゾウ アンサンブル ノ タンサク
• 広い構造アンサンブルの探索

抄録

Many proteins are considered to perform their functions by dynamic excursion to “other” conformations that deviate from the basic structure found in crystal. These “other” conformations have seldom become targets of detailed structural study. The on-line cell high pressure NMR technique developed at Kobe is the only available technique capable of producing “other” conformations of proteins and simultaneously reporting their structures at residue-specific resolution using multi-dimensional NMR spectroscopy. The principle is based on the recognition that the partial molar volume of a protein strongly depends on its conformational state. Examples are given from two proteins, basic pancreatic trypsin inhibitor and the Ras-binding domain of RalGEF.

収録刊行物

• 高圧力の科学と技術

  高圧力の科学と技術 10 (2), 88-94, 2000

  日本高圧力学会

キーワード

• high pressure NMR
• partial molar volume
• protein conformation
• pressure denaturation
• local unfolding
• multi-dimensional NMR
• structural fluctuation
• conformational ensemble

詳細情報

• CRID: 1390001204381135360
• NII論文ID: 10004668570
• NII書誌ID: AN10452913
• DOI: 10.4131/jshpreview.10.88
• COI: 1:CAS:528:DC%2BD3cXltFyrtbo%3D
• ISSN: 13481940
• ISSN: 0917639X
• ISSN: http://id.crossref.org/issn/0917639X
• NDL書誌ID: 5358044
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5358044
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可