高圧力下の酵素反応 [in Japanese] Enzyme Reaction under High Pressure [in Japanese]
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From the many studies in enzyme reactions under high pressure, the activation volume for the catalytic process and the reaction volume for the dissociation process of the Michaelis complex evaluated from the pressure dependence of the enzyme reaction are found to be powerful tools for studying the reaction mechanisms in these processes. The above volume changes not only clarify the enzyme reaction mechanism, but also may explain the relationship between the high catalytic efficiency/specificity and the reactaonmechanism. As an example of an enzyme reaction, the reaction mechanism of α-chymotrypsin (α-CHT) catalysis is discussed in terms of the volume change.
- The Review of High Pressure Science and Technology
The Review of High Pressure Science and Technology 10(2), 108-115, 2000-05-20
The Japan Society of High Pressure Science and Technology