A 3D Crystal of Bacteriorhodopsin Obtained by Successive Fusion of the Vesicular Assemblies.

DOI Web Site 16 References

Bibliographic Information

Other Title
  • 膜融合を利用したバクテリオロドプシンの結晶化法
  • ジッケンシツ マク ユウゴウ オ リヨウ シタ バクテリオロドプシン ノ ケッショウカホウ

Search this article

Abstract

Bacteriorhodopsin, the sole protein in the purple membrane of Halobacterium salinarium, functions as a light-driven proton pump. To obtain it's three-dimensional crystal, we have developed a new crystallization method which does not involve any step of complete destruction of the membrane. First, purple membrane was incubated at high temperature with neutral detergent and ammonium sulfate so that the membrane was converted into uniformly sized spherical vesicles. Then the vesicles were condensed at low temperature, where fusion of the vesicles produced a hexagonal crystal which is made up of planar membranes. Structural analysis showed that the trimeric structure of bacteriorhodopsin was retained in the crystal. The native lipid bound to a specific site in the protein was suggested to play an important role in maintaining the higher-order structure of the membrane.

Journal

References(16)*help

See more

Details 詳細情報について

Report a problem

Back to top