新規のビタミンB_<12>結合様式を持つジオールデヒドラターゼの立体構造と補酵素活性化機構 Crystal Structure of Diol Dehydratase Reveals a New Mode of B_<12> Binding and the Mechanism of Activation
Diol dehydratase is an adenosylcobalamin-dependant enzyme that catalyzes conversion from 1, 2-diol compounds to corresponding aldehydes. The crystal structure of the enzyme shows that B<SUB>12</SUB> compounds are incorporated with the DBI-on mode. The enzyme has a potassium ion at the active site, where the substrate coordinates to it. The structure of the enzyme complexed with adeninylpentylcobalamin reveals the activation mechanism of adenosylcobalamin.
日本結晶学会誌 42(4), 354-360, 2000-08-31
The Crystallographic Society of Japan