Production and Characterization of Monoclonal Antibodies Recognizing the Asymmetric Center of Optically Active Bufuralol and Its Metabolites

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A specific antibody has been produced for developing immunoaffinity chromatography of optically active bufuralol and its 1′-oxidized metabolites. Spleen cells from a BALB/c mouse, which had been immunized with the (1R)- or (1S)-1′-oxobufuralol O-carboxymethyl oxime-bovine serum albumin conjugate, were fused with P3/NS1/1-Ag4-1 myeloma cells. After screening by enzyme-linked immunosorbent assay (ELISA) using a β-galactosidase-labeled antigen, one for (1R)-bufuralol and three kinds for (1S)-bufuralol of antibody-secreting hybridoma clones were established. The monoclonal antibodies, Ab-R-12 and Ab-S-1,were determined to have favorable characteristics for preparing the immunosorbent, because each of them was specific for (1R)- or (1S)-bufuralols and its corresponding 1′-oxidized metabolites but showed significantly low cross-reactivities with their antipodes including 1′-oxidized metabolites.

収録刊行物

  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 16(1), 31-35, 2000-01-10

    社団法人 日本分析化学会

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各種コード

  • NII論文ID(NAID)
    10004704582
  • NII書誌ID(NCID)
    AA10500785
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09106340
  • NDL 記事登録ID
    4966599
  • NDL 雑誌分類
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL 請求記号
    Z54-F482
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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