Direct Determination of Platelet-Produced Thromboxane B2 in Human Serum by Column-Switching High-Performance Liquid Chromatography with Fluorescence Detection.
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- IWATA Tetsuharu
- Faculty of Pharmaceutical Sciences, Fukuoka University
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- HIROTA Kazuhito
- Faculty of Pharmaceutical Sciences, Fukuoka University
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- YAMAGUCHI Masatoshi
- Faculty of Pharmaceutical Sciences, Fukuoka University
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Abstract
A high-performance liquid chromatographic method for the direct determination of platelet-produced thromboxane B2 in human serum is described. The method is based on direct derivatization of the thromboxane B2 in human serum with 6,7-dimethoxy-1-methyl-2(1H)-quinoxalinone-3-propionyl carboxylic acid hydrazide. The derivatization reaction proceeds in aqueous solution (or serum sample) in the presence of pyridine and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide at 37°C. The resulting fluorescent derivative of thromboxane B2 is separated by column-switching chromatography using a first column (YMC-Pack C4) for the trap and clean-up of the derivative and a second column (YMC-Pack Ph) for complete separation of the derivative. The derivative is detected spectrofluorometrically at 445 nm with excitation at 367 nm. The detection limit (signal to noise ratio=3) for thromboxane B2 is 10 pmol (3.7 ng)/ml serum. The present method is highly sensitive and simple without any clean-up, such as in conventional liquid-liquid and solid-phase extraction.
Journal
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- Analytical Sciences
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Analytical Sciences 16 (1), 45-48, 2000
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390001204255723264
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- NII Article ID
- 10004704642
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- NII Book ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD3cXos1ekug%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 4966621
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- Web Site
- https://ndlsearch.ndl.go.jp/books/R000000004-I4966621
- https://link.springer.com/content/pdf/10.2116/analsci.16.45.pdf
- https://link.springer.com/article/10.2116/analsci.16.45/fulltext.html
- http://www.jstage.jst.go.jp/article/analsci/16/1/16_1_45/_pdf
- https://search.jamas.or.jp/link/ui/2000142752
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
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- CiNii Articles
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- Abstract License Flag
- Disallowed