High-Performance Liquid Chromatographic Quantification of Busulfan in Human Serum after Fluorescence Derivatization by 2-Naphthalenethiol
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- HARA Shuuji
- Faculty of Pharmaceutical Sciences, Fukuoka University
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- TSUCHIE Masahiko
- Faculty of Pharmaceutical Sciences, Fukuoka University
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- TSUJIOKA Riko
- Faculty of Pharmaceutical Sciences, Fukuoka University
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- KIMURA Masahiko
- Faculty of Pharmaceutical Sciences, Fukuoka University
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- FUJII Megumi
- Faculty of Pharmaceutical Sciences, Fukuoka University
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- KURODA Takeshi
- Faculty of Pharmaceutical Sciences, Fukuoka University
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- ONO Nobufumi
- Faculty of Pharmaceutical Sciences, Fukuoka University
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Abstract
A simple and highly sensitive fluorometric high-performance liquid chromatographic method was developed for the determination of busulfan in human serum. After busulfan and 1,6-bis(methanesulfonyloxy)hexane as an internal standard were extracted from serum with ethyl acetate, they were derivatized with 2-naphthalenethiol in an alkaline medium. The derivatives were separated by reversed-phase chromatography on a YMC-Pack C4 column with a mixture of methanol-0.1 M sodium acetate buffer (pH 7.0) (8:2, v/v) as a mobile phase, and were then detected spectrofluorometrically at 370 nm with excitation at 255 nm. Extraction and derivatization efficiencies were 73.9 - 75.1% and greater than 91.1%, respectively. The detection limit for busulfan added to serum was 2 ng (8 pmol) ml-1 serum (330 fmol on column) at a signal-to-noise ratio of three with a linear relationship over the 10 ng - 3.0 μg ml-1 (0.04 - 12 μM) concentration range. The accuracy and precision of this method were within 7.0% and 9.3% even at low concentration (10 ng ml-1). The within- and between-day variations were lower than 9.3% and 10.9%, respectively.
Journal
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- Analytical Sciences
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Analytical Sciences 16 (3), 287-291, 2000
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390001204258442880
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- NII Article ID
- 130004440235
- 10004705638
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- NII Book ID
- AA10500785
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- COI
- 1:CAS:528:DC%2BD3cXitFegurw%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 5312277
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- Web Site
- https://ndlsearch.ndl.go.jp/books/R000000004-I5312277
- https://link.springer.com/content/pdf/10.2116/analsci.16.287.pdf
- https://link.springer.com/article/10.2116/analsci.16.287/fulltext.html
- https://www.jstage.jst.go.jp/article/analsci/16/3/16_3_287/_pdf
- https://search.jamas.or.jp/link/ui/2000140338
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed