Determination of Total, Volatile and Acid Detergent Insoluble Nitrogen in Silage by Sequential Injection.

SILVA Fernando V., NOGUEIRA Ana RITA A., SOUZA Gilberto B., CRUZ Geraldo M.

doi:10.2116/analsci.16.361

Determination of Total, Volatile and Acid Detergent Insoluble Nitrogen in Silage by Sequential Injection.

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SILVA Fernando V.

Embrapa Pecuária Sudeste
NOGUEIRA Ana RITA A.

Embrapa Pecuária Sudeste
SOUZA Gilberto B.

Embrapa Pecuária Sudeste
CRUZ Geraldo M.

Embrapa Pecuária Sudeste

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This work proposes a sequential injection procedure to determine the total, volatile and acid detergent insoluble nitrogen content in silage. An ammonium tubular selective electrode coupled to a sequential injection manifold was used as detector to accomplish the potentiometric determinations made after on-line ammonia diffusion in alkaline medium through a Teflon membrane and NH₃/NH₄⁺ conversion. Different sample treatments, taking into account the species to be analyzed, were adopted to apply the proposed procedure to silage samples. An analytical frequency of 30 samples h^-1 and a detection limit of 3.0 mg N l^-1 were observed when 300 µl of 3.0 mol l^-1 NaOH solution and a sample volume of 150 µl were employed. The use of the proposed procedure allowed the automation of the determinations, low sample and reagent consumption and small waste production, emphasizing the versatility and potentiality of the flow methods when applied to animal nutrition laboratories.