6-[N-(3-Propionohydrazino)thioureido]benzo[g]-phthalazine-1, 4(2H, 3H)-dione as a Derivatization Reagent for Fatty Acids in High-Performance Liquid Chromatography with Chemiluminescence Detection

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6-[N-(3-Propionohydrazino)thioureido]benzo[g]-phthalazine-1,4(2H,3H)-dione was found to be a sensitive chemilumi-nescence (CL) derivatization reagent for fatty acids in high-performance liquid chromatography. The reagent readily reacted selectively with fatty acids at room temperature in the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodi-imide and pyridine in aqueous solution to form the corresponding acid hydrazide derivatives, which produce chemiluminescence by reacting with hydrogen peroxide in the presence of potassium hexacyanoferrate(III) in an alkaline solution. The CL derivatives of seven fatty acids (myristic, linolenic, palmitoleic, linoleic, palmitic, oleic and stearic acids) were separated within 55 min by reversed-phase (C18) liquid chromatography with isocratic elution, and were detected with CL detection after mixing with the oxidizing reagents. Calibration curves were linear up to at least 66.7 pmol per 20 µl injection volume (r=0.996 to 0.999). The detection limits (signal-to-noise ratio=3) for the acids were 11.8 - 64.4 fmol for an injection volume of 20 µl.

収録刊行物

  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry  

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 15(10), 937-941, 1999-10-10 

    社団法人 日本分析化学会

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各種コード

  • NII論文ID(NAID)
    10004707759
  • NII書誌ID(NCID)
    AA10500785
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09106340
  • NDL 記事登録ID
    4872779
  • NDL 雑誌分類
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL 請求記号
    Z54-F482
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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