Quantitative Determination of Pregnenolone 3-Sulfate in Rat Brains Using Liquid Chromatography/Electrospray Ionization-Mass Spectrometry
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A method for the quantitative determination of pregnenolone 3-sulfate (PS) in rat brains has been developed using liquid chromatography/electrospray ionization-mass spectrometry (LC/ESI-MS). The PS fraction was obtained from the rat brain homogenate by solid-phase extraction and ion-exchange chromatography. After the derivatization with 4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole, PS was determined using LC/ESI-tandem MS along with the standard addition method. This method was applied to the quantitative determination of this steroid in the brains of Wistar strain rats, most of which showed a much lower amount than that previously reported.
- Analytical Sciences
Analytical Sciences 15(10), 951-955, 1999-10-10
The Japan Society for Analytical Chemistry