Quantitative Determination of Pregnenolone 3-Sulfate in Rat Brains Using Liquid Chromatography/Electrospray Ionization-Mass Spectrometry.

• MITAMURA Kuniko

  Faculty of Pharmaceutical Sciences, Kanazawa University

• YATERA Misako

  Faculty of Pharmaceutical Sciences, Kanazawa University

• SHIMADA Kazutake

  Faculty of Pharmaceutical Sciences, Kanazawa University

Abstract

A method for the quantitative determination of pregnenolone 3-sulfate (PS) in rat brains has been developed using liquid chromatography/electrospray ionization-mass spectrometry (LC/ESI-MS). The PS fraction was obtained from the rat brain homogenate by solid-phase extraction and ion-exchange chromatography. After the derivatization with 4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole, PS was determined using LC/ESI-tandem MS along with the standard addition method. This method was applied to the quantitative determination of this steroid in the brains of Wistar strain rats, most of which showed a much lower amount than that previously reported.

Journal

• Analytical Sciences

  Analytical Sciences 15 (10), 951-955, 1999

  The Japan Society for Analytical Chemistry

Keywords

• Analytical Chemistry

Details

• CRID: 1390001204256287232
• NII Article ID: 10004707785
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.15.951
• COI: 1:CAS:528:DyaK1MXms1agtr0%3D
• ISSN: 13482246
• ISSN: 09106340
• ISSN: http://id.crossref.org/issn/09106340
• NDL BIB ID: 4872804
• Web Site: http://hdl.handle.net/2297/3899
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4872804
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.15.951.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.15.951/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/15/10/15_10_951/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2000119687
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed