Determination of Ascorbate Concentration in a Raw Leaf with Electron Spin Resonance Spectroscopy

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The concentration of ascorbate in a raw leaf was determined by monitoring a nitroxyl radical reduction with X-band ESR. One piperidine nitroxyl derivative, 2,2,6,6-tetramethyl-4-hydroxypiperidine-1-oxyl (TEMPOL), was reduced according to first-order kinetics by more than 5-fold excess of ascorbate. The observed reduction rate was found to be proportional to ascorbate concentration in phosphate buffer (0.1 mol/dm3 , pH 7.4) at 25°C. The reduction of TEMPOL in leaf homogenate of sprouts of radish was measured; it was found to obey first-order kinetics. The ascorbate concentration in leaf was estimated from observed reduction rate and found to be virtually identical with that from a visible absorption spectrophotometric analysis. The features of our ESR method for sensitivity, procedure and measuring time were discussed with comparisons from other methods. The results indicated that the determination of ascorbate in a raw leaf by ESR spectroscopy was simple and effective.

収録刊行物

  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 15(10), 973-977, 1999-10-10

    社団法人 日本分析化学会

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各種コード

  • NII論文ID(NAID)
    10004707843
  • NII書誌ID(NCID)
    AA10500785
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09106340
  • NDL 記事登録ID
    4872847
  • NDL 雑誌分類
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL 請求記号
    Z54-F482
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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