Identification and genetic mapping of RAPD markers linked to the densonucleosis refractoriness gene, nsd-2, in the silkworm, Bombyx mori

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著者

    • Abe Hiroaki ABE Hiroaki
    • Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology
    • SHIMADA Toru
    • Graduate School of Agriculture and Life Sciences, The University of Tokyo
    • GOMI Sumiko Jun
    • Cellular Signaling Laboratory RIKEN(The Institute of Physical and Chemical Research)
    • OHBAYASHI Fumi
    • Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology
    • OSHIKI Toshikazu
    • Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology

抄録

In the silkworm, <i>Bombyx mori</i>, nonsusceptibility to <i>B. mori</i> densonucleosis virus type-2 (BmDNV-2) is controlled by a recessive gene, <i>nsd-2</i> (nonsusceptibility to DNV-2). We investigated the genetic linkage between two random-amplified polymorphic DNA (RAPD) markers and the +<sup>nsd-2</sup> gene. Initially, we constructed the JSD-2 strain (<i>nsd-2</i>/+), which is congenic to strain J137 (<i>nsd-2</i>/<i>nsd-2</i>) with respect to the chromosome containing the +<sup>nsd-2</sup> gene, starting with a female of strain J137 and a male of strain C137 (+<sup>nsd-2</sup>/+ <sup>nsd-2</sup>). Genomic DNAs were compared between infected individuals of the JSD-2 strain and J137 by a polymerase chain reaction (PCR) with 700 arbitrary 10-mer primers. Two RAPD markers (OPH19R and OPP01R) linked to the +<sup>nsd-2</sup> gene were found. For the crossing-over experiment, a female of J137 was crossed with a male (<i>nsd-2</i>/+) of JSD-2. Segregation analysis showed that the most closely linked RAPD marker (OPP01R) was mapped 4.7 cM distant from +<sup>nsd-2</sup>.<br>

収録刊行物

  • Genes & genetic systems  

    Genes & genetic systems 75(2), 93-96, 2000-04 

    The Genetics Society of Japan

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各種コード

  • NII論文ID(NAID)
    10004717854
  • NII書誌ID(NCID)
    AA11077421
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417568
  • NDL 記事登録ID
    5428614
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z53-W539
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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