Characterization of Early Follicular cDNA Library Suggests Evidence for Genetic Polymorphisms in the Inbred Strain C108 of Bombyx mori

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Recent work towards the completion of a saturated molecular genetic linkage map for the lepidopteran silkworm, <i>Bombyx mori</i> (<i>n</i>=28), has provided evidence for existing polymorphisms in the inbred strain C108. Two inbred parental strains, p50 and C108, were crossed to produce the F1 (P/C) hybrid offspring. The populations used in this project were comprised of a combination of 29 F2 (F1 <i>x</i> F1) and 31 reciprocal backcross (P/C <i>x</i> C/C, P/C <i>x</i> P/P) progeny. All restriction fragment length polymorphisms (RFLPs) for the initial analysis were hybridized with anonymous probes derived from a random early follicular cDNA (Rcf) library from <i>Bombyx</i>. A total of 19 Rcf probes were selected as showing scorable codominant polymorphic patterns when screened against F2 and backcross DNAs digested with the restriction enzymes <i>Eco</i>RI, <i>Hin</i>dIII, or <i>Pst</i>I, and Southern blotted to nylon membranes for hybridization. Of the newly reported Rcf probes, 7 (37%) were characterized as producing `simple' polymorphic patterns, while 12 (63%) were characterized as producing `complex' polymorphic patterns. Further characterization of the complex patterns subdivided this group into two general classes: polymorphisms that contained an additional allele, and multiple bands that contained an easily scored two banded polymorphism. Because the extra allele class was limited to the (P/C <i>x</i> C/C) backcross progeny, it is suggested that the inbred parental strain C108 harbors polymorphic loci that are inherited in a simple Mendelian fashion. A genetic analysis discussing plausible origins and maintenance of these polymorphisms is presented.<br>

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  • Genes & genetic systems  

    Genes & genetic systems 75(2), 105-113, 2000-04 

    The Genetics Society of Japan

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各種コード

  • NII論文ID(NAID)
    10004717911
  • NII書誌ID(NCID)
    AA11077421
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417568
  • NDL 記事登録ID
    5428657
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z53-W539
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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