Effects of the Herbicide Molinate on Russell's Viper Venom Clotting Time and Chromogenic Substrate Assay for Factor X in the Plasma of Common Carp, Cyprinus carpio.

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  • 除草剤モリネートがコイ血しょうのRVV凝固時間および第X因子測定用基質発色に及ぼす影響
  • 除草剤モリネートがコイ血漿のRVV凝固時間および第X因子測定用基質発色に及ぼす影響

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Abstract

The hemostatic disorder in the common carp induced by exposure to the herbicide molinate and the preventive effect of menadione dimethylpyrimidinol bisulfite (MPB) were examined by means of clotting time and chromogenic substrate assay using S-2337. Fish were arranged in three groups of 7 animals each. The fish in the first group were exposed to molinate alone at 0.32 ppm, those in the second group to 0.32 ppm of molinate and 32.4 ppb of MPB, and those in the third group served as the control. Measurements of activated partial thromboplastin time (APTT), prothrombin time (PT), Russell's viper venom time (RVVT) and chromogenic substrate assay (CSA) were performed at the start, and after 7 and 12 days of exposure. Prolongation of APTT, PT, and RVVT, and a decrease in absorbance in the CSA were observed after 7 days of exposure to molinate. Thereafter, the prolongation of PT became more pronounced than that of APTT and RVVT, indicating that dysfunction of the extrinsic pathway was more severe than that of intrinsic and common pathways. MPB was highly effective in preventing the prolongation of clotting time and the decrease in absorbance in the CSA.

Journal

  • Aquaculture Science

    Aquaculture Science 43 (1), 153-156, 1995

    Japanese Society for Aquaculture Science

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