ワムシの高密度間引き培養におけるアンモニアと懸濁物およびワムシ現存量の変化

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タイトル別名
  • Changes in the Concentrations of Ammonia and Particulate Organic Matter and Rotifer Biomass in High Density Semi-Continuous Culture.
  • ワムシ ノ コウ ミツド マビキ バイヨウ ニ オケル アンモニア ト ケンダ

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For the reliable process management of high density culture of marine rotifer Brachionus rotundiformis, it is very important to know the quantitative behavior of rotifer biomass, the concentrations of particulate organic matters including nutritional organic matters for rotifer, and total ammonia (NH4-N) . In this study, changes in dry weight, PV (packed volume) and population of rotifer, PV of particulate organic matters and concentrations of NH4-N in high density semicontinuous culture were investigated. High density culture was performed by supplying oxygen from a generator, controlling the pH at 6.7 and feeding condensed Chlorella regularis at a feed rate of 4.2 kg/day with a peristaltic pump. Filtration unit was also used to remove the excess amount of particulate organic matters (mainly bacterial flocs and films) from culture medium. Total amount of accumulated Chlorella (Rn, g) in the culture tank was expressed with the feed rate of Chlorella (S, g/day), culture day (n) and harvest rate (T) as follows.<BR>Rn= {1- (1-T) n} / {1- (1-T) } ⋅S<BR>The amount of NH4-N produced (Yn, g) and the concentration of NH4-N in culture medium (An, ppm) were estimated by following exprimental formulas. where a is the initial ammonia concentration (ppm) and V is working volume (kl) .<BR>Yn= 0.0326 Rn (r=0.95, p<0.01)<BR>An=a⋅ (1-T) n+Yn/V<BR>The result of partial correlation analysis for the PVs of rotifer, wastes layer, and microbial layer and culture day indicated that the wastes layer was utilized by rotifer as feed but microbial layer was not utilized. As the result of multiple regression analysis, it was proved that the measurement of PV was more reliable among the methods for quantitative determination of rotifer biomass. The measurement of PV can be easily performed by the centrifugation of culture medium, therefore PV monitoring will be very useful for on-line management of high density culture of rotifer. In the semicontinuous high density culture of 20 days, the feed conversion rate of Chlorella to rotifer was found to be 23.5% (w/w) . Dry weight and PV per rotifer individual were estimated to be 1.6 × 10-7 g and 9.2 × 10-7 ml, respectively, and those per rotifer egg were 0.9 × 10-7 g and 4.9 × 10-7 ml, respectively.

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  • 水産増殖

    水産増殖 46 (2), 183-192, 1998

    日本水産増殖学会

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