Simple Method for Detection of Clostridium botulinum Type A to F Neurotoxin Genes by Ploymerase Chain Reaction
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- TAKESHI Kouichi
- Hokkaido Institute of Public Health
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- FUJINAGA Yukako
- Department of Bacteriology, Okayama University Medical School
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- INOUE Kaoru
- Department of Bacteriology, Okayama University Medical School
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- NAKAJIMA Hiroshi
- Department of Bacteriology, Okayama University Medical School
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- OGUMA Keiji
- Department of Bacteriology, Okayama University Medical School
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- UENO Tetsuya
- Research Laboratory, Toa Pharmaceutical Company Ltd.
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- SUNAGAWA Hiroyuki
- Hokkaido Institute of Public Health
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- OHYAMA Tohru
- Hokkaido Institute of Public Health
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抄録
A polymerase chain reaction (PCR)-based method was established to detect each type of neurotoxin genes of Clostridium botulinum types A to F by employing the oligonucleotide primer sets corresponding to special regions of the light chains of the neurotoxins. In this procedure, the PCR products were easily confirmed by restriction enzyme digestion profiles, and as little as 2.5pg of template DNAs from toxigenic strains could be detected. The specific PCR products were obtained from toxigenic C. botulinum types A to F, a type E toxin-producing C. butyricum strain, and a type F toxin-producing C. baratii strain, but no PCR product was detected in nontoxigenic strains of C. botulinum and other clostridial species. The neurotoxin genes were also detected in food products of a seasoned dry salmon and a fermented fish (Izushi) which had caused type E outbreaks of botulism. Therefore, it is concluded that this PCR-based detection method can be used for the rapid diagnosis of botulism.
収録刊行物
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- Microbiology and immunology
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Microbiology and immunology 40 (1), 5-11, 1996-01-20
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詳細情報
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- CRID
- 1572261549108850432
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- NII論文ID
- 10004786679
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- NII書誌ID
- AA00738350
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles