Determination of Asperulosidic Acid and Deacetylasperulosidic Acid in Rat Plasma After Administration of Morinda citrifolia Juice
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- WADA Mitsuhiro
- School of Pharmaceutical Sciences, Kyushu University of Health and Welfare Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University
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- YAMASAKI Yutaro
- Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University
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- NAKAMURA Shinichi
- School of Pharmaceutical Sciences, Kyushu University of Health and Welfare
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- TAKADA Makoto
- Fuculty of Pharmaceutical Sciences, Nagasaki International University
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- KAWAKAMI Shigeru
- Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University
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- NAKASHIMA Kenichiro
- Fuculty of Pharmaceutical Sciences, Nagasaki International University
書誌事項
- タイトル別名
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- Determination of Asperulosidic Acid and Deacetylasperulosidic Acid in Rat Plasma After Administration of <i>Morinda citrifolia</i> Juice
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<p>Determination of asperulosidic acid (AA) and deacetylasperulosidic acid (DAA) in rat plasma after administration of Morinda citrifolia juice by HPLC-UV detection was described. Deproteinized rat plasma (200 L) with MeOH for the HPLC analysis was injected. AA and DAA was separated on ZIC®-HILIC (250×4.6 mm, i.d., 5 μm) with a mixture of CH3CN/MeOH/0.1% formic acid aqueous solution (=70:25:5, v/v/v) at a flow rate of 0.75 mL/min. The absorbance of eluate was monitored at 235 nm. Under this condition, the separation of AA and DAA was achieved within 25 min. The calibration curves using plasma spiked with standards indicated good linearity (r≥0.996) in the range of 0.8-20 g/mL for AA and 4-100 μg/mL for DAA, respectively. The limits of quantitation for AA and DAA at a signal-to-noise ratio of 10 were 0.3 and 1.1 μg/mL, respectively. The validation parameters of the method such as recovery (94.9-101.7 %), precisions (less than 7.1 % for intra-day and less than 9.8 % for inter-day) and accuracy (95.0-101.7 %) were acceptable. The analytes in rat plasma were stable after three freeze-thaw cycles or storage at room temperature for up to 6 h. Furthermore, monitoring of AA and DAA after administration of the Morinda citrifolia juice in which AA and DAA were determined, was successfully demonstrated. This is the first report to determine AA and DAA in rat plasma after administration of Morinda citrifolia juice.</p>
収録刊行物
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- CHROMATOGRAPHY
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CHROMATOGRAPHY 37 (3), 141-145, 2016
クロマトグラフィー科学会
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詳細情報 詳細情報について
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- CRID
- 1390001205745118080
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- NII論文ID
- 130005277438
- 10004787228
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- NII書誌ID
- AA1137755X
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- ISSN
- 13483315
- 13428284
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- NDL書誌ID
- 027713152
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可