Structure-Function Relationship of Ribonuclease T<SUB>1</SUB> Based on Molecular Structure

• Kojima Masaki

  School of Life Science, Tokyo University of Pharmacy and Life Science

• Tanokura Masaru

  Graduate School of Agricultural and Life Sciences, University of Tokyo

• Takahashi Kenji

  School of Life Science, Tokyo University of Pharmacy and Life Science

Abstract

Ribonuclease T₁ (RNase T₁) is a guanyloribonuclease consisting of 104 amino acid residues. It has been subjected to a number of efforts to elucidate the catalytic and substrate-recognition mechanisms and molecular stability. Since the first crystal structure of RNase T₁ was determined in 1982, many studies on the structure-function relationships based on the three-dimensional molecular structure have been carried out. We also studied the conformations of RNase T₁ and its mutants/derivatives, mainly by NMR and molecular dynamics simulation. In this review, we discuss the structure-function relationship of RNase T₁ especially in terms of the three-dimensional structure.

Journal

• bioimages

  bioimages 8 (2), 45-55, 2000

  Bioimaging Society

Keywords

• ribonuclease T<SUB>1</SUB>
• guanylic acid
• active site
• molecular dynamics

Details

• CRID: 1390845712992686208
• NII Article ID: 10004826415
• NII Book ID: AA11084187
• DOI: 10.11169/bioimages.8.45
• ISSN: 09192719
• Text Lang: en
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed