Application of Cationic Liposomes to Introduction of Monoclonal Antibodies into Live Cells
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- Ohuchi Takashi
- Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo
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- Fukushima Takahiro
- Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo
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- Maruyama Kiyotoshi
- Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo
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- Arai Takao
- Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo
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抄録
For investigation of phenomena in live cells by bioimaging, it is very important to introduce probes into cells without significant damage. Cationic liposomes are widely used to introduce genes into live culture cells. In this study, the transfection method using cationic liposomes was applied to introduction of monoclonal antibodies into cells cultured on 96-well plastic culture plates. First, we examined the introduction efficiency of an anti-β-tubulin monoclonal antibody into cells of PC12, primary cultured glial cells derived from E17 rat brain and cells of rat fibroblast cell strain 3Yl by cationic liposomes. Among four commercially available liposomes, lipofectamine, lipofectin, cellfectin, and DMRIE-C reagents, lipofectamine was most effective in all cases. Second, we demonstrated that this method was useful for observation of microtubules in these live cells. Third, we confirmed that lipofectamine caused no significant damage to viability of the cells. These data suggest that cationic liposomes are also a useful vehicle to introduce monoclonal antibodies as molecular probes for detection of native structure in live cells.
収録刊行物
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- bioimages
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bioimages 8 (2), 57-64, 2000
日本バイオイメージング学会
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詳細情報 詳細情報について
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- CRID
- 1390564238017637120
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- NII論文ID
- 10004826484
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- NII書誌ID
- AA11084187
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- ISSN
- 09192719
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可