誘電泳動クロマトグラフィーによる生体分子の分離 Separation of Biomolecules using Dielectrophoretic chromatography

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Dielectrophoresis (DEP) refers to the motion of particles that results from the interaction between a non-uniform external field and the induced dipole moment. Under an extremely high field-gradient created in microfabricated electrode systems, objects with molecular dimensions, such as DNA, can be attracted and trapped. Since the DEP force is stronger for large objects, biomolecules can be separated by the difference in their molecular size. In this paper, the electric field-flow separator, which we call DEP chromatography, is developed and tested. The device consists of a microelectrode array for dielectrophoretic trapping of biomolecules, which is covered by a lid having a microfabricated fluid passage. Water solutions of λ-DNA (48.5kbp) and an oligonucleotide (22 nucleotides) are fed to the device, separately or as a mixture. Under the field strength of 1MV/m at IMHz, λ-DNA is collected onto the electrode array, while the oligonucleotide is not, thus enabling the separation between λ-DNA and the oligonucleotide. During the experiment, dependence of the DEP collection on the molecular concentration is observed. The interpretation for this phenomenon is the aggregate formation at high molecular concentrations due to the attractive force between induced dipole moments on the molecules.

収録刊行物

  • 電気学会論文誌. E, センサ・マイクロマシン準部門誌 = The transactions of the Institute of Electrical Engineers of Japan. A publication of Sensors and Micromachines Society  

    電気学会論文誌. E, センサ・マイクロマシン準部門誌 = The transactions of the Institute of Electrical Engineers of Japan. A publication of Sensors and Micromachines Society 119(10), 454-459, 1999-10 

    The Institute of Electrical Engineers of Japan

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各種コード

  • NII論文ID(NAID)
    10004834269
  • NII書誌ID(NCID)
    AN1052634X
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    13418939
  • NDL 記事登録ID
    4866311
  • NDL 雑誌分類
    ZN31(科学技術--電気工学・電気機械工業)
  • NDL 請求記号
    Z16-B380
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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