Development of Microfabricated Capillary Array Electrophoresis Chip and Direct Observation of Dynamics of DNA Molecules Migrating in Microchannels

• Ueda Masanori

  The University of Tokushima

• Tabata Osamu

  Ritsumeikan University

• Baba Yoshinobu

  The University of Tokushima

抄録

We have examined the LIGA process for the microfabrication of capillary array electrophoresis (CAE) chip using synchrotron radiation lithography in polymethylmethacrylate (PMMA) substrates and developed novel type microfabricated CAE chip. Laser-induced fluorescence detection system for the CAE chip has been constructed by the modification of laser confocal fluorescence microscopy. DNA molecules labeled by intercalating fluorescent dye were detected during migrating in the array of microchannels and conformational dynamics of single DNA molecule in microchannels filled with linear polyacrylamide under electric field was observed by this system to elucidate the separation mechanism of DNA and to optimize the separation conditions for DNA analysis. Based on this observation, we demonstrated that linear polyacrylamide is suitable for the rapid separation of large DNA fragments less than 166 kbp by microfabricated CAE chip without pulsed electric field.

収録刊行物

• 電気学会論文誌Ｅ（センサ・マイクロマシン部門誌）

  電気学会論文誌Ｅ（センサ・マイクロマシン部門誌） 119 (10), 460-463, 1999

  一般社団法人 電気学会

キーワード

• capillary array electrophoresis
• microfabrication
• LIGA process
• DNA analysis
• laser induced fluorescence detection

詳細情報

• CRID: 1390001204462359808
• NII論文ID: 10004834276
• NII書誌ID: AN1052634X
• DOI: 10.1541/ieejsmas.119.460
• ISSN: 13475525
• ISSN: 13418939
• ISSN: http://id.crossref.org/issn/13418939
• NDL書誌ID: 4866317
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4866317
• Web Site: http://www.jstage.jst.go.jp/article/ieejsmas1995/119/10/119_10_460/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可