Microfabricated Chips for Capillary Electrophoresis on Quartz Glass Substrates

• Nishimoto Takahiro

  Shimadzu Corp.

• Abe Hirohisa

  Shimadzu Corp.

• Kanai Masaki

  Shimadzu Corp.

• Fujiyama Yoichi

  Shimadzu Corp.

• Nakanishi Hiroaki

  Shimadzu Corp.

• Arai Akihiro

  Shimadzu Corp.

Bibliographic Information

Other Title

• 電気泳動用石英ガラス製マイクロチップの開発
• デンキ エイドウヨウ セキエイ ガラスセイ マイクロチップ ノ カイハツ

Abstract

Microchips for capillary electrophoresis have been fabricated in small size on quartz glass substrates. Microchannels with smooth sidewalls are etched by applying adopted etching condition. To fabricate quartz glass chips, we applied a novel bonding method with dilute hydrofluoric acid solution. Using fabricated microchips, we show that they permit to perform quantitative sample injection (several 10pl or less), effective separation (the number of theoretical plates is over 2, 500) and high speed analysis (_??_30 sec). In order to improve the detection sensotivity of UV absorbance method, we have developped the chips which have optical slit, and confirmed that the absorbance unit was improved about ten times.

Journal

• IEEJ Transactions on Sensors and Micromachines

  IEEJ Transactions on Sensors and Micromachines 119 (10), 476-481, 1999

  The Institute of Electrical Engineers of Japan

Keywords

• キャピラリー電気泳動
• 石英ガラス
• マイクロマシニング

Details

• CRID: 1390001204461530624
• NII Article ID: 10004834308
• NII Article ID: 10005310808
• NII Book ID: AN1052634X
• DOI: 10.1541/ieejsmas.119.476
• ISSN: 13475525
• ISSN: 13418939
• ISSN: http://id.crossref.org/issn/13418939
• NDL BIB ID: 4866325
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4866325
• Web Site: http://www.jstage.jst.go.jp/article/ieejsmas1995/119/10/119_10_476/_pdf
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed