Amperometric glucose sensor using glassy carbon electrode anodized in 1, 5-pentanediol as the base transducer
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- Mizutani Fumio
- National Institute of Bioscience and Human-Technology
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- Yabuki Soichi
- National Institute of Bioscience and Human-Technology
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An amperometric glucose-sensing electrode was prepared by immobilizing glucose oxidase (GOx) on a polyion complex-modified glassy carbon (GC) electrode. First, a GC electrode was anodized in a 1, 5-pentanediol solution to introduce carboxyl groups on the electrode surface. Next, aqueous solutions of poly-L-lysine and poly (4-styrenesulfonate) were successively placed on the electrode and allowed to dry. Finally a GOx layer was formed on the poly-L-lysine/poly(4-styrenesulfonate)-complex layer by crosslinking the enzyme by the addition of glutaraldehyde. The use of the thin bilayer system with inner, polyion complex membrane, which showed permselectivity based on the solute size with molecular cut-off of ca. 100, brought about high performance characteristics, such as rapid response and low interference level, to the glucose-sensing electrode. The enzyme electrode was useful for the measurement of glucose in beverages involving vitamin C-enriched drinks.
収録刊行物
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- 電気学会論文誌E(センサ・マイクロマシン部門誌)
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電気学会論文誌E(センサ・マイクロマシン部門誌) 119 (11), 554-559, 1999
一般社団法人 電気学会
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詳細情報 詳細情報について
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- CRID
- 1390001204462608000
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- NII論文ID
- 10004834530
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- NII書誌ID
- AN1052634X
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- ISSN
- 13475525
- 13418939
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- NDL書誌ID
- 4892735
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可