DNA固定化カーボンフェルトをリアクターとするDNA結合性薬物のフロー型バイオセンシング Flow-through Biosensing of DNA-binding Drugs using DNA-Immobilized Carbon Felt as a Reactor
Oxygen electrode-based flow-type amperometric mutant sensor was proposed using DNA-immobilized carbon felt as a sensing element for DNA-binding drugs. Catalytic activity of immobilized DNA-Cu (II) complex for air oxidation of aminoethanthiol (AET) was reversibly inhibited by intercalation with DNA binding drugs and antibiotics. Kinetic measurements indicate that quinacrine (QN) competitively inhibits the DNA-Cu (II) complex-catalyzed oxidation of AET. These phenomena could be utilized for development of novel flow-type biosensing system for DNA-binding drugs consisting with immobilized DNA-Cu (II) reactor and oxygen electrode detector. The peak-shape current responses were observed for various DNA-binding drugs, and the magnitude and the shape of the responses depend considerably on the kinds and concentrations of DNA-binding drugs.
- 電気学会論文誌. E, センサ・マイクロマシン準部門誌 = The transactions of the Institute of Electrical Engineers of Japan. A publication of Sensors and Micromachines Society
電気学会論文誌. E, センサ・マイクロマシン準部門誌 = The transactions of the Institute of Electrical Engineers of Japan. A publication of Sensors and Micromachines Society 119(11), 571-575, 1999-11
The Institute of Electrical Engineers of Japan