SPR免疫センサによるメタンフェタミン(覚醒剤)の高感度検出とその抗原抗体反応解析 Highly sensitive detection of methamphetamine(stimulant drug) by using SPR-based immunosensor and kinetic study of the immunoreaction on sensor element

この論文にアクセスする

この論文をさがす

著者

抄録

Highly sensitive detection of methamphetamine (MA) was realized by using an immunosensor based on surface plasmon resonance (SPR) phenomena. A methamphetamine-bovine serum albumin (MA-BSA) conjugate was prepared as an antigen, and it was immobilized on the Au thin film of sensor-chip. Introduction of MA into the antibody solution was found to decrease the incident angle shift sharply because of the inhibition effect of MA. Association constants between anti-MA antibody and MA as well as anti-MA antibody and MA-BSA were evaluated from the Langmuir isotherm adsorption model. The association constants between anti-MA antibody and MA or MA-BSA were estimated as 5.68×10<sup>6</sup>M<sup>-1</sup>, 2.92×10<sup>7</sup> M<sup>-1</sup> respectively. The association rate constant and dissociation rate constant were also estimated from typical transient responses to antibody solutions.

収録刊行物

  • 電気学会論文誌. E, センサ・マイクロマシン準部門誌 = The transactions of the Institute of Electrical Engineers of Japan. A publication of Sensors and Micromachines Society

    電気学会論文誌. E, センサ・マイクロマシン準部門誌 = The transactions of the Institute of Electrical Engineers of Japan. A publication of Sensors and Micromachines Society 119(11), 581-586, 1999-11

    The Institute of Electrical Engineers of Japan

参考文献:  12件中 1-12件 を表示

被引用文献:  3件中 1-3件 を表示

各種コード

  • NII論文ID(NAID)
    10004834619
  • NII書誌ID(NCID)
    AN1052634X
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    13418939
  • NDL 記事登録ID
    4892822
  • NDL 雑誌分類
    ZN31(科学技術--電気工学・電気機械工業)
  • NDL 請求記号
    Z16-B380
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
ページトップへ