SPR免疫センサによるメタンフェタミン(覚醒剤)の高感度検出とその抗原抗体反応解析 [in Japanese] Highly sensitive detection of methamphetamine(stimulant drug) by using SPR-based immunosensor and kinetic study of the immunoreaction on sensor element [in Japanese]
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Highly sensitive detection of methamphetamine (MA) was realized by using an immunosensor based on surface plasmon resonance (SPR) phenomena. A methamphetamine-bovine serum albumin (MA-BSA) conjugate was prepared as an antigen, and it was immobilized on the Au thin film of sensor-chip. Introduction of MA into the antibody solution was found to decrease the incident angle shift sharply because of the inhibition effect of MA. Association constants between anti-MA antibody and MA as well as anti-MA antibody and MA-BSA were evaluated from the Langmuir isotherm adsorption model. The association constants between anti-MA antibody and MA or MA-BSA were estimated as 5.68×10<sup>6</sup>M<sup>-1</sup>, 2.92×10<sup>7</sup> M<sup>-1</sup> respectively. The association rate constant and dissociation rate constant were also estimated from typical transient responses to antibody solutions.
- The Journal of the Institute of Electrical Engineers of Japan
The Journal of the Institute of Electrical Engineers of Japan 119(11), 581-586, 1999-11
The Institute of Electrical Engineers of Japan